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2013 R&D 100 Winner
Phenomenex Phree Phospholipid Removal PlatesPhospholipids are a source of negative chromatographic effects in LC/MS/MS analysis. Bioanalytical sample preparation approaches such as protein precipitation removes only proteins not phospholipids, resulting in samples that are prone to these negative effects.

Phenomenex’s Phree Phospholipid Removal Plates remove proteins and phospholipids by following the same procedure used to perform protein precipitation. The plate is composed of a medical-grade polypropylene 96-well well plate which contains Phree sorbent, a high-affinity sorbent for phospholipid, and a specialized frit system that is designed to withhold solvent and sample until pressure is applied. The system is also designed to easily process tissue samples without clogging, and allows precipitating solvent and sample to be held within the wells of the plate for up to 20 min, more than enough time for protein precipitation to occur. Once pressure is applied, the precipitated sample passes through the frit and into the Phree sorbent which selectively removes phospholipids. The Removal Plates act as a filter during this process and removes precipitated proteins as the sample passes through the frits.

Technology
Phospholipid removal plates

Developers
Phenomenex Inc.


Development Team

Phenomenex's Phree Phospholipid Removal Plates development team. Back row (l-r): Mo Kazemi, Yibo Guo, A. Carl Sanchez, Erica Pike Safan. Front row (l-r): Stuart Kushon, Shahana Wahab Huq, Arthur R. Dixon.

 










The Phree Phospholipid Removal Plates Development Team from Phenomenex Inc.
Stuart Kushon, Principal Developer
Arthur R. Dixon
Yibo Guo
Shahana Wahab Huq
Mo Kazemi
Erica Pike Safan
A. Carl Sanchez
Shiferaw Tekle

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