2013 R&D 100 Winner
Conventional assays based on UV-Vis spectroscopy rely on absorbance by tryptophane and tyrosine residues with minor contribution from cysteine, and therefore have limited utility. However, EMD Millipore’s Direct Detect is a mid-infrared (MIR)-based spectroscopy system for protein quantitation that does not rely on amino acid composition, dye-binding properties or redox potential. The system employs a hydrophilic polytetrafluoroethylene (PTFE) membrane designed to be transparent in most of the infrared spectral region and enables application of biomolecule solutions directly onto the membrane. The system requires minimal sample consumption, and its accuracy and precision are comparable with results obtained by amino acid analysis. Because the system relies on MIR-based detection of biomolecules, users can obtain accurate and reproducible protein quantitation in the presence of reducing agents and detergents. As a result, the Direct Detect system can measure protein concentrations from 0.25 to 5 mg/mL within seconds, without any bio- or immunochemical staining, directly from samples, including buffered solutions.

Mid-infrared (MIR)-based spectroscopy system for protein quantitation

EMD Millipore

Development Team

The Direct Detect Development Team from EMD Millipore
Joseph Lento, Principal Developer
Ivona Strug, Principal Developer
Christopher Utzat, Principal Developer