Univ. of Utah researchers devised a way to watch newly forming AIDS virus particles emerging or “budding” from infected human cells without interfering with the process. The method shows a protein named ALIX gets involved during the final stages of virus replication, not earlier, as was believed previously.
Researchers at the National Physical Laboratory and the London Centre for Nanotechnology have determined the structure of DNA from measurements on a single molecule using atomic force microscopy (AFM), and found significant variations in the well-known double helix.
Fluorescent proteins have helped researchers open doors to countless molecular imaging applications and deepened our understanding of biological processes. Without fluorescence, advancements in oncology, drug discovery and any field that requires single-cell to whole-body imaging would be substantially limited.
A key step in the decades-long mystery of the HIV lifecycle was uncovered using what formerly was thought of as only a supplementary x-ray technique for structural biology. This advances study of HIV as well as highlights a powerful tool to obtain currently unobtainable high-resolution structural determination and characterization of RNA molecules.
Researchers have recently developed a unique technology to help physicians perform ultrasound-guided procedures involving needle placement. The new imaging technology, created by Clear Guide Medical, allows physicians to plan needle entry and a precise line to the target before the needle ever enters the patient’s organ or tissue. The result is more efficient, less damaging, and less stressful needle-placement procedures for patients.
An interdisciplinary team of scientists in Belgium has developed a new technique to examine how proteins interact with each other at the level of a single HIV viral particle. The technique allows scientists to study the life-threatening virus in detail and makes screening potential anti-HIV drugs quicker and more efficient. The technique can also be used to study other diseases.
Launched in 2013, the national BRAIN Initiative aims to revolutionize our understanding of cognition by mapping the activity of every neuron in the human brain, revealing how brain circuits interact to create memories, learn new skills and interpret the world around us. Before that can happen, neuroscientists need new tools that will let them probe the brain more deeply and in greater detail.
Medical nanoparticles need to be eventually eliminated from the body after they complete their task. Researchers have developed a new method to analyze and characterize this process of nanoparticle “disassembly”, as a necessary step in translating nanoparticles into clinical use. The technique involves the use of Förster resonance energy transfer, or FRET, as a sort of molecular ruler to measure distance at small scales.
Researchers in the U.K. have applied “soft-touch” atomic force microscopy to large, irregularly arranged and individual DNA molecules. In this form of microscopy, a miniature probe is used to feel the surface of the molecules one by one, rather than seeing them. In this way they have determined the structure of DNA from measurements on a single molecule, and found that the structure is more irregular than previously thought.
Researchers have developed an ultrasound device that could help identify arterial plaque that is at high risk of breaking off and causing heart attack or stroke. At issue is the plaque that builds up in arteries as we age. Some types of plaque are deemed “vulnerable,” meaning that they are more likely to detach from the artery wall and cause heart attack or stroke.
Unlike healthy cells, cancer cells thrive when deprived of oxygen. Tumors in low-oxygen environments tend to be more resistant to therapy and spread more aggressively to other parts of the body. Measuring tumors’ oxygen levels could help doctors make decisions about treatments, but there’s currently no way to make such measurements. However, a new sensor developed at Massachusetts Institute of Technology could change that.
A team of scientists, led by physicist Amir Yacoby of Harvard Univ., has developed a magnetic resonance imaging (MRI) system that can produce nanoscale images, and may one day allow researchers to peer into the atomic structure of individual molecules. Though not yet precise enough to capture atomic-scale images of a single molecule, the system already has been used to capture images of single electron spins.
When considering potential drug delivery vehicles, liposomes are an important option and have already been approved for use with a number of therapeutic formulations. Liposomes are comprised of phospholipids and may be single- or multi-layered, can be produced in different sizes and have a hydrophilic interior and hydrophobic shell. They are biodegradable, non-toxic and capable of encapsulating both hydrophilic and hydrophobic materials.
Biological samples bend light in unpredictable ways, returning difficult-to-interpret information to the microscope. Using a form of adaptive optics, Janelia Farm Research Campus scientists have developed a microscopy technique that can rapidly correct for distortions and sharpen high-resolution images over large volumes of tissue.
Picking out a face in the crowd is a complicated task: Your brain has to retrieve the memory of the face you’re seeking, then hold it in place while scanning the crowd, paying special attention to finding a match. A new study reveals how the brain achieves this type of focused attention on faces or other objects.
Our fascination with mummies never gets old. Now the British Museum is using the latest technology to unwrap their ancient mysteries. Scientists at the museum have used CT scans and sophisticated imaging software to go beneath the bandages, revealing skin, bones, preserved internal organs, and in one case a brain-scooping rod left inside a skull by embalmers. The findings go on display next month in an exhibition.
Under the microscope, they glow like streetlights, forming tidy rows that follow the striations of muscle tissue. They are mitochondria—the powerhouses of cells—and researchers at the Univ. of Virginia School of Medicine have created a method to illuminate and understand them in living creatures like never before.
Chemists have settled the debate about a fundamental question that is relevant to the conversion of one color into another and demonstrated how to influence the efficiency of this process by changing the refractive index around the material.
A new brain imaging study in Australia found a “stop mechanism” that determined brain signals telling the individual to stop drinking water when no longer thirsty. The study, which used magnetic resonance imaging, also gauged the brain effects of drinking more water than required.
In order to track the movements of biological particles in a cell, scientists at Heidelberg Univ. and the German Cancer Research Center have developed a powerful analysis method for live cell microscopy images. This so-called probabilistic particle tracking method is automatic, computer-based and can be used for time-resolved 2-D and 3-D microscopy image data.
Using only data from an fMRI scan, researchers led by a Yale Univ. undergraduate have accurately reconstructed images of human faces as viewed by other people. The increased level of sophistication of fMRI scans has already enabled scientists to use data from brain scans taken as individuals view scenes and predict whether a subject was, for instance, viewing a beach or city scene, an animal or a building.
Doctors commonly use MRI to diagnose tumors, damage from stroke and many other medical conditions. Neuroscientists also rely on it as a research tool for identifying parts of the brain that carry out different cognitive functions. Now, a team of biological engineers at Massachusetts Institute of Technology is trying to adapt MRI to a much smaller scale.
In 2007, Massachusetts Institute of Technology scientists developed a type of microscopy that allowed them to detail the interior of a living cell in 3-D, without adding any fluorescent markers or other labels. This technique also revealed key properties, such as the cells’ density. Now the researchers have adapted that method so they can image cells as they flow through a tiny microfluidic channel.
When cancers become advanced, tumor cells from the primary tumor can enter the bloodstream and cause metastasis at another organ with deadly effect. While researching the biological implications of CTC spread, Creatv MicroTech researchers found a group of previously unreported cells associated with primary cancer spread. These macrophage-like cells could serve as biomarkers.
Skeletal muscles are built from small contractile units, the sarcomeres. Many of these sarcomeres are connected in a well-ordered series to form myofibrils that span from one muscle end to the other. Scientists recently identified a key mechanism how this basic muscle architecture is built during development.