Biophysicists at Rice Univ. have used a miniscule machine, a protease called an FtsH-AAA hexameric peptidase, as a model to test calculations that combine genetic and structural data. Their goal is to solve one of the most compelling mysteries in biology: how proteins perform the regulatory mechanisms in cells upon which life depends.
If you’ve ever suffered the misery of food poisoning from a bacterium like Salmonella, then your cells have been on the receiving end of “nanoinjectors”, microscopic spikes made from proteins through which pathogens secrete effector proteins into human host cells, causing infection. Researchers are using advanced nuclear magnetic resonance spectrometry to unlock the structure of these injector, which are built from 20 different proteins.
Particle counters are used in a wide variety of industries. Researchers in North Carolina have developed a new thermal technique that counts and measures the size of particles, but is less expensive than light-based techniques. It can also be used on a wider array of materials than electricity-based techniques.
Incomplete or infrequent water quality data can give an inaccurate picture of what’s happening in water resources. Using UV-Vis spectrometers that can rapidly collect data, researchers have developed a new technique o allow researchers and natural resource managers to collect significantly more information on water quality to better inform policy decisions.
A pathway to more effective and efficient synthesis of pharmaceutical drugs and other flow reactor chemical products has been opened by a study in which, for the first time, the catalytic reactivity inside a microreactor was mapped in high resolution from start-to-finish. The results not only provided a better understanding of the chemistry behind the catalytic reactions, they also revealed opportunities for optimization.
The time and cost of sequencing an entire human genome has plummeted, but analyzing three billion base pairs from a single genome can take many months. However, a Univ. of Chicago-based team working with Beagle, one of the world's fastest supercomputers devoted to life sciences, reports that genome analysis can be radically accelerated. The Argonne National Laboratory computer is able to analyze 240 full genomes in about two days.
Researchers at NIST have developed a new method for accurately measuring a key process governing a wide variety of cellular functions that may become the basis for a health checkup for living cells. The NIST technique measures changes in a living cell's internal redox (reduction-oxidation) potential, a chemistry concept that expresses the favorability of reactions in which molecules or atoms either gain or lose electrons.
Optogenetics allows scientists to control neurons’ electrical activity with light by engineering them to express light-sensitive proteins, called opsins. Most opsins respond to light in the blue-green range. Now, a team has discovered an opsin that is sensitive to red light, which allows researchers to independently control the activity of two populations of neurons at once, enabling much more complex studies of brain function.
Stem cell research has been breaking ground in new application areas over the past few years, and it’s poised for even greater growth as more companies and organizations realize the potential. In the next decade, cell-based therapies will become increasingly common for cancer, immunological disorders, cardiac failure and other conditions.
Progress often requires change. For protein-based diagnostics, multiplexed assays and detection of protein isoforms will drive the adoption of a new strategy for diagnostic testing, called immuno-MS. Enzyme-linked immunosorbent assays (ELISA) have become the standard for antibody-based diagnostic tests in clinical settings. ELISAs provide specific detection of biomarkers through use of antibodies which target specific epitopes on antigens.
Medical laboratory test results provide physicians with vital information needed for accurate diagnosis, treatment and monitoring of patients. An estimated 60 to 70% of all decisions regarding a patient’s diagnosis and treatment, hospital admission and discharge are based on laboratory test results.
Laboratory automation techniques are commonplace, as they improve the accuracy and repeatability of laboratory operations, reduce human error in these operations and reduce cost of these operations. Defined as the use of technology to streamline or substitute manual manipulation of equipment and processes, laboratory automation offers solutions for enhancing workflows in various research laboratory environments.
As interest and investment in biopharmaceuticals grows, the pressure to innovate and rapidly deliver new therapies increases. While many avenues may be pursued, the high cost of developing biological molecules increases the need to advance only those therapies with the greatest likelihood of becoming manufacturable, efficacious, safe and profitable products.
Awareness of the benefits of gravimetric sample preparation has increased significantly over the past couple of years. Recognition of this state-of-the-art technology by industry organizations such as the United States Pharmacopeia (USP) has supported this trend. A recent revision to USP chapter 1251 “Weighing on an Analytical Balance” included a detailed description of the steps involved in gravimetric dosing for sample preparation.
The benefits of flow cytometry are well known. The popular technique allows researchers to explore data on a cell-by-cell basis, as opposed to other analysis methods which only offer population-based or averaged information. In addition, flow cytometry can give users absolute percentages of what each marker or dye is reporting.
A team of engineers from the Univ. of California, Los Angeles has developed a smartphone attachment and application to test water for the presence of mercury, a toxic heavy metal. The new platform could significantly reduce the time and cost of the testing, and it could be particularly useful in regions with limited technological resources.
An array of tiny diving boards can perform the Olympian feat of identifying many strains of salmonella at once. The novel biosensor developed by scientists at Rice Univ. in collaboration with colleagues in Thailand and Ireland may make the detection of pathogens much faster and easier for food-manufacturing plants.
An international research team led by Mount Sinai Heart at Icahn School of Medicine at Mount Sinai, is testing its novel sugar-based tracer contrast agent to be used with positron emission tomography (PET) imaging to help in the hunt for dangerous inflammation and high-risk vulnerable atherosclerotic plaque inside vessel walls that causes acute heart attacks and strokes.
The ability to determine protein stoichiometry and monitor the ratio of protein types allows scientists to see the difference between a properly functioning cell and a diseased cell. Photoactivation fluorescences studies have resulted in undercounting of proteins, however, leading a science group to recently establish new methodology for determining protein stoichiometry.
Researchers at Oregon State Univ. have discovered novel compounds produced by certain types of chemical reactions, such as those found in grilling meat, that are hundreds of times more mutagenic than their parent compounds which are known carcinogens. These compounds were not previously known to exist, and raise additional concerns about the health impacts of heavily polluted urban air or dietary exposure.
G protein-coupled receptors (GPCR) are a highly diverse group of membrane proteins that mediate cellular communication and are thought to be prominent drug targets. A group of researchers from Arizona State Univ. are part of a larger team reports that they have been successful in imaging at room temperature the structure of GPCR with the use of an x-ray free-electron laser.
An international research team has produced a high-quality genome sequence of a Neanderthal woman from a toe bone found in 2010 by Russian archaeologists. The genome will allow detailed insights into the relationships and population history of the Neanderthals and other extinct hominin groups.
New collaborative work from computational biologists in Massachusetts and California combines computational and experimental approaches to identify biologically meaningful RNA folds. The work could open the door to a better understanding of RNA machinery, which includes the ribosome, microRNAs and riboswitches, and long noncoding RNAs whose diverse functions are only beginning to be understood.
In a sort of biological "spooky action at a distance," water in a cell slows down in the tightest confines between proteins and develops the ability to affect other proteins much farther away, Univ. of Michigan researchers have discovered. The finding could provide insights into how and why proteins clump together in diseases such as Alzheimer's and Parkinson's.
Until recently, the microscopic study of complex membrane proteins has been restricted due to limitations of “force microscopes” that are available to researchers and the one-dimensional results these microscopes reveal. Now, researchers at the Univ. of Missouri have built a 3-D microscope that will yield unparalleled information on membrane proteins and how they interact in cells. The innovation could speed up drug development.