Target Enrichment System for Next-Generation Sequencers
Agilent Technologies Inc. has introduced a faster version of the HaloPlex target enrichment system for desktop sequencers. The protocol that previously required approximately 24 hrs can now be run in less than six, according to the company.
The new HaloPlex requires 200 ng of input DNA and is compatible with FFPE samples. The product comes with 96 indexes enabling sequencing of several samples in parallel.
The technology combines the speed and specificity of polymerase chain-reaction systems with the scalability and capture-size flexibility of in-solution hybridization formats. The library prep-free single tube protocol overcomes the target-number limitations of multiplex PCR due to cross-hybridization issues, reduces sample prep time, and is compatible with Illumina MiSeq desktop sequencers, as well as HiSeq and GAIIx sequencers. The workflow is simpler and faster than hybrid capture and microfluidics PCR approaches.
Agilent Technologies Inc., www.agilent.com