UltraPerformance Convergence Chromatography
Waters Corporation introduced its ACQUITY UPC2 System at Pittcon 2012. The system expands the boundaries of reversed phase liquid chromatography (LC) and gas chromatography (GC) separations and offers a replacement option for normal phase chromatography applications. Employing the principle of UltraPerformance Convergence Chromatography (UPC2), the analytical system gives laboratories a tool for tackling tough-to-analyze compounds including hydrophobic and chiral compounds, lipids, thermally labile samples, and polymers.
Compressed carbon dioxide, the primary mobile phase for UPC2, offers advantages over liquid mobile phases or carrier gases that are used with LC and GC. One of those advantages is carbon dioxide alone, or in combination with a co-solvent, is a low viscosity mobile phase that achieves higher diffusion rates and enhanced mass transfer than liquids used in HPLC. Another advantage is, when compared to GC, carbon dioxide alone is a mobile phase that allows separations to occur at a much lower temperature.
Along with sub two-micron particle column chemistries, the system gives scientists the ability to vary mobile phase strength, pressure, and temperature. With this ability to fine tune the resolving power and selectivity of the system, scientists can exercise better control over the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diasteriomeric mixtures.
Waters Corporation, www.waters.com