Multiphoton confocal microscopes (LSM 7 series) from Carl Zeiss now allow the simultaneous use of two NLO lasers or one laser with an optical parametric oscillator (OPO). Both components are integrated and expand the functionality of the multiphoton systems.
In dual-laser systems, different laser wavelengths simultaneously excite fluorescent dyes or proteins. Without time loss, users can image specimens with one wavelength and manipulate them in multiphoton mode with another. The automatic free beam adjustment gives the system a high degree of stability and reproducibility and ensures exact overlay of the two excitation beams.
An OPO increases the excitation range of multiphoton microscopy up to 1,300 nm, which covers the absorption peak of red fluorescent proteins. This long-wave excitation enables specimen protection. The high light intensities of the OPO lasers interact with specific structures in the tissue, resulting in a doubling or tripling of the oscillation frequency.
Carl Zeiss Microscopy LLC, www.zeiss.com/micro