Agilent Technologies Inc. announced it has expanded its family of sub-2 µm particle 300 Angstrom pore columns for reversed-phase liquid chromatography with ZORBAX 300SB-C3 and 300-Diphenyl 1.8 µm columns.
The columns extend the benefits of reversed-phase biomolecule separations to ultra-high-performance liquid chromatography (UHPLC). The C3 phase broadens the selectivity and improves peak shape and recovery options for larger proteins, including antibodies. The diphenyl phase offers additional selectivity through pi-pi interactions with aromatic amino acids in the primary structure.
The 1.8 µm particle size and 300 Angstrom pore size bring UHPLC efficiency, resolution, and quantitation to reversed-phase protein separations. Additionally, the columns are stable up to 1200 bar. Agilent's StableBond technology for the C18, C8, and C3 columns, and the end-capped diphenyl with Pursuit chemistry, deliver symmetrical peaks—even at low pH—when using trifluoroacetic or formic acid mobile phase modifiers. Column life is not compromised.
Agilent Technologies Inc., www.agilent.com