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Uncovering the secrets of tularemia

Tue, 02/18/2014 - 8:26am
Stephen P Wampler, Lawrence Livermore National Laboratory

Shown is the x-ray crystal structure of a putative virulence factor protein from Francisella tularensis. The crystals were grown at LLNL, and the x-ray data were collected at the Advanced Photon Source at Argonne National Laboratory. The image was generated with the Chimera molecular graphics package developed by the Univ. of California, San Francisco. Image: Geoffrey Feld /LLNLTularemia is endemic in the northeastern U.S., and is considered to be a risk to biosecurity—much like anthrax or smallpox—because it has already been weaponized in various regions of the world.

At the 58th Annual Biophysical Society Meeting, Geoffrey Feld, a postdoctoral researcher in Lawrence Livermore National Laboratory (LLNL)'s Physical & Life Sciences Directorate, has described his work to uncover the secrets of the bacterium Francisella tularensis, which causes tularemia, also known as "rabbit fever."

"Despite its importance for both public health and biodefense, F. tularensis pathogenesis isn't entirely understood, nor do we fully understand how the organism persists in the environment," Feld explained.

Previous efforts, funded by both the National Institutes of Health and LLNL, demonstrated that amoebae may serve as a potential reservoir for the bacteria in nature. "Specifically, we demonstrated that amoebae exposed to fully virulent F. tularensis rapidly form cysts—dormant, metabolically inactive cells—that allow the amoebae to survive unfavorable conditions," said Amy Rasley, the research team leader.

This rapid formation of amoebic cysts, referred to as REP by the researchers for Rapid Encystment Phenotype, was induced by F. tularensis in the laboratory and was required for the long-term survival of the bacteria. Further exploration identified secreted F. tularensis proteins, which are responsible for the observed encystment of the amoeba.

In the new work, Feld and colleagues characterized two of these REP proteins—called REP24 and REP34—and began to describe their functions based on their 3-D crystal structures.

A surprise finding was that these proteins resembled "proteases," which are proteins that cut other proteins in a specific manner. "Our preliminary data indicate that F. tularensis bacteria lacking these proteins are diminished in their ability to infect or survive in human immune cells, which indicates that these proteins may also contribute to F. tularensis virulence," Feld said.

Rasley and colleagues believe that careful characterization of these two novel F. tularensis proteins may shed light on how this organism persists in the environment and causes disease.

"Ultimately, this type of research could inform efforts to combat the disease, although there is much work to do. Currently, we don't know the protein targets in the host—amoeba, human, etc.—that the REP proteins act on, nor do we know the mechanism by which the proteins could help F. tularensis survive in the environment or cause disease," Feld said.

"Once these questions are elucidated, a broader understanding of environmental persistence and pathogenesis might lead to better diagnostics and/or novel countermeasures to combat tularemia," he added.

Source: Lawrence Livermore National Laboratory

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