Greater Peak Detection for HPLC
2014 R&D 100 Winner
HPLC and UHPLC are ubiquitous laboratory tools with enormous libraries of established separation methods for a vast array of compounds, deployed in diverse fields. The concern and challenge for any analyst considering a new technology for their application involves the effort required to adapt, transfer or recreate an already established method in a way that preserves the utility of the original method. Excellims Corp.’s IA3100 integrated, compact high-performance ion mobility spectrometry (HPIMS) detector for high-performance liquid chromatography (HPLC) systems provides complete separation and detection of complex mixtures, particularly when adding the IA3100 capabilities to existent LC hardware, and can complement or serve as an replacement for an end user’s present LC detector.
The core technology of the IA3100 uses advanced, HPIMS to separate ions introduced via electrospray ionization from the corresponding HPLC eluent. HPIMS demonstrates higher performance over conventional IMS by offering ion mobility base separation having resolving power comparable to HPLC. Capitalizing on Excellims’ patented instrument design, the most important technology advancement is that the HPIMS achieves 60 to 120 resolving power, comparable to the HPLC/UHPLC method coupled to it and two to 10 times better than conventional IMS, maintaining the fidelity of the original chromatographic separation. The HPIMS separation also occurs in just milliseconds with total acquisition times of seconds per sample, so the detection is only limited by the elution time from the HPLC or UHPLC.
|Excellims Corp.'s IA3100 HPIMS development team. Back row (l-r): Adam Craichen, Mark Osgood and Robert Jackson. Front row (l-r): Eugenie Hainsworth, Anthony Midey, Carol Moraff and Ching Wu.|
The IA3100 High Performance Ion Mobility Spectrometer Development Team from Excellims Corp.
Ching Wu, Principal Developer