Brute strength gives gonorrhea the edge
April 23, 2008
Researchers from the Univ. of Arizona and Columbia Univ. have discovered that tiny filaments on bacteria can bundle together and pull with forces far stronger than experts had previously thought possible.
The team of researchers, including Magdalene “Maggie” So, a member of the BIO5 Institute and the department of immunobiology in the Univ. of Arizona College of Medicine, studied Type IV pili—or filaments—on the surface of Neisseria gonorrhoeae, the bacterium that causes the infectious disease gonorrhea. The research results help them understand the role that Type IV pili play in initiating a variety of infectious diseases—including tuberculosis—and how retracting pili allow bacteria to crawl and to exchange genes with each other.
When a bundle of Type IV pili retracts, it pulls with a force in the nanoNewton range, which is 10 times the force of a single retracting filament. The study demonstrates the power and cooperative nature of the nanomotors that cause Type IV pili to retract.
“The motor that causes these filaments to pull is one of the strongest nanomotors known in biology,” So says.
In previous studies, the same group of investigators measured single filament retraction forces in the 50 to 100 picoNewton range. This force allows the bacterium to move an object 10,000 times its own body weight. Retraction forces from a bundle are roughly 10 times higher, allowing the bacterium to move objects 100,000 times its body weight.
Pilus retraction forces are an important factor in how N. gonorrhoeae starts an infection. So, who has studied these microbes for more than 20 years, says N. gonorrhoeae communicates with a human cell by pulling on it. These pulling forces perturb the normal circuitry of the cell. As a result, the infected cell is fooled into lowering its defenses against the infecting microbe.
So said that the team of investigators came up with a new method to measure the tremendous forces applied by retracting pili. They allow bacteria to sit on a dense brushwork of tiny elastic pillars. The pili attach to these pillars. When pili retract, they bend the pillars. By measuring how the pillars bend, the investigators calculate the retraction forces.
An article about the research, titled “Cooperative Retraction of Bundled Type IV Pili Enables Nanonewton Force Generation,” was published in the latest issue of PLoS Biology. Authors of the PLoS Biology article are Nicolas Biais and Mike Sheetz, Columbia Univ.; Benoit Ladoux, Université Paris 7; So and Dustin Higashi, both from the Univ. of Arizona.
The article is available here: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2292754
SOURCE: Univ. of Arizona
Researchers from the Univ. of Arizona and Columbia Univ. have discovered that tiny filaments on bacteria can bundle together and pull with forces far stronger than experts had previously thought possible.
|
When a bundle of Type IV pili retracts, it pulls with a force in the nanoNewton range, which is 10 times the force of a single retracting filament. The study demonstrates the power and cooperative nature of the nanomotors that cause Type IV pili to retract.
“The motor that causes these filaments to pull is one of the strongest nanomotors known in biology,” So says.
In previous studies, the same group of investigators measured single filament retraction forces in the 50 to 100 picoNewton range. This force allows the bacterium to move an object 10,000 times its own body weight. Retraction forces from a bundle are roughly 10 times higher, allowing the bacterium to move objects 100,000 times its body weight.
Pilus retraction forces are an important factor in how N. gonorrhoeae starts an infection. So, who has studied these microbes for more than 20 years, says N. gonorrhoeae communicates with a human cell by pulling on it. These pulling forces perturb the normal circuitry of the cell. As a result, the infected cell is fooled into lowering its defenses against the infecting microbe.
|
An article about the research, titled “Cooperative Retraction of Bundled Type IV Pili Enables Nanonewton Force Generation,” was published in the latest issue of PLoS Biology. Authors of the PLoS Biology article are Nicolas Biais and Mike Sheetz, Columbia Univ.; Benoit Ladoux, Université Paris 7; So and Dustin Higashi, both from the Univ. of Arizona.
The article is available here: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2292754
SOURCE: Univ. of Arizona
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