2010 R&D 100 Winner
The ONIX Microfluidic Perfusion System from CellASIC Corp., San Leandro, Calif., provides a controllable environment for living cells needed for time-lapsed analysis on standard microscopes. The core technology is in the microfluidic plates, which feature microchambers for cell health and reservoirs for exposure solutions. The cell culture chambers are designed to match the low shear stress and high nutrient/gas transport found in nature.
Cells are introduced into a well in 5 µl quantities using a pipette. The corresponding outlet well is emptied. Capillary flow pulls the cells into microchambers in one minute. Once cells are in a chamber, continuous perfusion of culture medium allows the cells to be maintained more than three days.
The Control System maintains fluidic control of the plates. A vacuum line forms a seal between the manifold and plate; eight pressure lines allow independent control of flow inlets. The tenth line delivers CO2 or other gas mixtures.
Technology
Microfluidic cell perfusion system
Developers
CellASIC Corp.
Development Team
|
| Top row (l-r): Philip Lee, Paul Hung. Middle row: Brandon Miller, Daniel Kim, Molly Allen, Jessie Ho, Eric Phoumthipphavong. Bottom row: Alex Mok, Julia Olsen-Claire, Terry Gaige, Cecilia Chiu, Cindy Chen. |
The ONIX Microfluidic Perfusion System Development Team from CellASIC:
Molly Allen
Cindy Chen.
Cecilia Chiu
Terry Gaige
Jessie Ho
Paul Hung
Daniel Kim
Philip Lee
Brandon Miller
Alex Mok
Julia Olsen-Claire
Eric Phoumthipphavong
The ONIX Microfluidic Perfusion System from CellASIC Corp. provides a controllable environment for living cells needed for time-lapsed analysis on standard microscopes.
